yqeV [2019-08-16 10:33:04]

tRNA methylthiotransferase

Locus: BSU_25430

Isoelectric point: 5.24

Molecular weight: 51.49 kDa

Protein length: 451 aa

Gene length: 1356 bp

Function: tRNA maturation

Product: tRNA methylthiotransferase

Essential: no

Synonyms: yqeV

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
• Lifestyles → Coping with stress → Heat shock proteins

Gene

Coordinates: 2,621,677 2,623,032

The protein

Catalyzed reaction/ biological activity

• modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed
• [sulfur carrier]-SH + AH₂ + N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H⁺ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• methylthiotransferase family (with YmcB, according to UniProt)

Paralogous protein(s)

• YmcB

Cofactors

• 2 Fe-S clusters PubMed

Structure

• 4JC0 (from Thermotoga maritima, 29% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • HrcA: repression, PubMed, in HrcA regulon

  Regulation

  • induced by heat shock (HrcA) PubMed

Biological materials

Mutant

• MGNA-C496 (yqeV::erm), available at the NBRP B. subtilis, Japan
• BKE25430 (yqeV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA
• BKK25430 (yqeV::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA

References

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