yqeV [2019-08-16 10:33:04]

tRNA methylthiotransferase

locus: BSU_25430

pI: 5.24

mw: 51.49 kDa

protein length: 451 aa

gene length: 1356 bp

function: tRNA maturation

product: tRNA methylthiotransferase

essential: no

synonyms: yqeV

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

Gene

Coordinates: 2,621,677 2,623,032

The protein

Catalyzed reaction/ biological activity

• modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA [Pubmed|27913733,20472640]
• [sulfur carrier]-SH + AH₂ + N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 2 S-adenosyl-L-methionine --> 2-methylsulfanyl-N⁶-L-threonylcarbamoyladenosine³⁷ in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H⁺ + L-methionine + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• methylthiotransferase family (with [protein|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|YmcB], according to UniProt)

Paralogous protein(s)

• [protein|7C9F99FF1BCDB0E1CFB0AFE959C7F16A99562E18|YmcB]

[SW|Cofactors]

• 2 Fe-S clusters [Pubmed|20584901]

Structure

• [PDB|4JC0] (from ''Thermotoga maritima'', 29% identity) [Pubmed|23542644]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
  description: [Pubmed|9023197]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1339421], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|1339421], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]

  regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]

Biological materials

Mutant

• MGNA-C496 (yqeV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2494 NBRP B. subtilis, Japan]
• BKE25430 ([gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA
• BKK25430 ([gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGTATGGAAAGCAACAGTTG, downstream forward: _UP4_TAACCTAAAAAATCGGTGCA

References

• 10383760,9023197,7592421,20472640,20584901,27913733,23542644